O projekcie

Tytuł:

Nowatorskie metody inżynierii tkankowej wspomagające gojenie i regenerację ścięgien i więzadeł

 

Abstract:

Despite advances in treatment of tendon and ligament injuries, which are the most common musculoskeletal disorders that clinicians address daily, the question of optimal treatment is still unanswered. The main aim of the project is to develop a novel Scaffold-based Tissue engineering Approaches to healing and Regeneration of Tendons and ligaments (START). We hypothesize that in situ guided personalized tissue engineering, where smart, biodegradable patient/case–specific scaffold is used to provide biomimetic 3D micro- and nano-environments, and delivery required molecules and stem cells, would enhance the tendon/ligament regeneration. The bioactive scaffold will also recruit and stimulate endogenous stem/progenitor cells to assist tissue formation. Additionally, the scaffold, due to its special construction, will allow for continuous in situ modulation of regeneration by providing directly to injury site the stem cells, cytokines, and by applying mechanical stimulation to the tissue throughout whole time of therapy. The modulation will depend on results of in situ biomarkers analyzes. The START will also require the establishment of new non-invasive imaging and analytical techniques for in situ monitoring of regeneration processes. Implementing such multidisciplinary strategies for tissue engineering should greatly enhance the efficacy of treatment of tendon/ligament disorders.

The main goal of the project is to develop a Novel Scaffold-based Tissue Engineering Approach to Healing and Regeneration of Tendons and Ligaments (START). We hypothesize that in situ guided personalized tissue engineering, where smart, biodegradable patient/case–specific scaffold is used to provide biomimetic 3D micro- and nano-environments, and delivery of required molecules and stem cells, at the time of surgical repair and during rehabilitation after surgery, would enhance the tendon/ligament regeneration.

To achieve the main goal of the project specific aims are also defined:

Aim 1. To gain fundamental knowledge on how soft tissue forms and behaves to define the main “biological players” (cells and bioactive cues) in tissue healing and regeneration, and to define the requirements for best scaffold chemical composition and structure, and mechanical properties;

Aim 2. To develop smart, bioactive and biodegradable patient/case–specific scaffolds applied with minimally invasive technique, which will delivery required molecules and stem cells and provide biomimetic and bioactive 3D micro- and nano-environments for endogenous and transplanted cells;

Aim 3. To develop a method for continuous in situ modulation of regeneration by providing on demand, based on in situ biomarkers analyzes and depended on the stage of tissue repair, directly to injury site the stem cells, cytokines and mechanical stimuli throughout whole time of therapy;

Aim 4. To develop a 3D non-invasive method for monitoring of in vivo tissue healing and regeneration progress;

Aim 5. To preclinical evaluate and demonstrate the effectiveness of the proposed methods in vitro models and in appropriate animal model;

Aim 6. To analyze how to translate a Novel Scaffold-based Tissue Engineering Approaches to Healing and Regeneration of Tendons and Ligaments (START) to the clinical (human) safety trials.

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